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ISOLATION OLIGOSACCHARIDES FROM GEMBILI (DIOSCOREA ESCULENTA LOUR. BURKILL) AS PREBIOTICS
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Abstract. Oligosaccharides is a potential prebiotic and used as an alternative diet therapy for
diabetes mellitus. Gembili (Dioscorea esculenta (Lour.) Burkill) is known as functional food
that contains oligosaccharides. This study was done to isolate oligosaccharides from gembili
and evaluate its prebiotics potential. Oligosaccharides was extracted and analysed by High
Performance Liquid Chromatography (HPLC). Potential of prebiotics was analysed using
faecal samples from healthy volunteers in anaerobic condition at certain time interval (0, 24,
and 48 hours) and bacterial growth (Bifidobacteria and Lactobacillus as probiotic bacteria,
Bacteroides and Clostridium as patogen bacteria), prebiotic index were analysed. Short Chain
Fatty Acid (SCFA) production (acetic acid, propionic acid, and butyric acid) analyzed using
HPLC. The results showed that oligosaccharides from gembili contains lactulose (0,231 %);
inulin (2,541 %) and rafinose (1,485 %). Prebiotic index increased from 0,86 ± 0,20 (24 hours
incubation) to 1,12 ± 0,05 (48 hours). This oligosaccharides increased probiotics and decreased
patogen bacteria. It could be concluded that oligosaccharides from gembili were potential as
prebiotics.
Detail Information
| Item Type |
Other
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|---|---|
| Penulis |
Serafica Btari Christiyani K, S.Si., M.Sc - Personal Name
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| Student ID | |
| Dosen Pembimbing | |
| Penguji | |
| Kode Prodi PDDIKTI | |
| Edisi |
Published
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| Departement | |
| Kontributor | |
| Bahasa |
English
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| Penerbit | : IOP Publishing., 2019 |
| Edisi |
Published
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| Subyek | |
| No Panggil | |
| Copyright | |
| Doi |